The lab states fixation will result in the dyes to leak in the cells. I think the assumption is usually that fixation might destruction mobile membranes. I did locate a paper which describes fixation employing methanol or acetic acid, immediately after staining that has a DCFDA by-product. DCFDA may be https://2-fdck-hcl61272.qodsblog.com/28039696/examine-this-report-on-2-fdck-hcl